The 50 Mile follows the same Hennepin Canal State Trail as the 100 Mile from Sinnissippi Park in Sterling, but finishes at Lock 17 near Wyanet rather than continuing to Colona. No pacing is allowed. Finishers earn a medal shaped like one of the canal's bridges.
The course is 'mostly rustic': dirt, chip-and-seal (a tar with pressed rock), crushed limestone, and a scattering of paved sections, plus a few blocks of road at the very start in Sterling.
The Hennepin Hundred is Illinois' only point-to-point, all-trail ultramarathon, running from Sterling, Illinois on the Rock River to Colona, Illinois near the Mississippi, along the historic Hennepin Canal. The 50- and 100-mile courses follow the Hennepin Canal State Trail across historic locks and lift bridges, past autumn corn, farms and small towns of northwest Illinois. It has been a Western States 100 qualifying race every year since 2019, and a course many runners choose for a PR or a first 100-mile finish.
The race states it has been a Western States 100 qualifying race every year since its 2019 running. UltraSignup's results index for this event goes back to 2015, with no results year listed for 2020.
| Mile | Station | Cutoff | Crew access | Bag | Pacer | Med | To next |
|---|---|---|---|---|---|---|---|
| 0.00 | Sinnissippi Park (Start) | ? | ? | ? | ? | ? | 5.30 |
| 5.30 | Aid Station 1 | 8:50am | · | · | · | ? | 5.50 |
| 10.80 | Aid Station 2 | 10:35am | · | · | · | ? | 4.10 |
| 14.90 | Aid Station 3 | 11:50am | ● | · | · | ? | 5.50 |
| 20.40 | Aid Station 4 | 1:10pm | · | ● | · | ? | 2.70 |
| 23.10 | Aid Station 5 | 1:55pm | ● | · | · | ? | 4.20 |
| 27.30 | Aid Station 6 | 3:15pm | · | · | · | ? | 4.80 |
| 32.10 | Aid Station 7 | ? | ● | ● | · | ? | 6.60 |
| 38.70 | Aid Station 8 | ? | ● | · | · | ? | 5.60 |
| 44.30 | Aid Station 9 | ? | · | · | · | ? | 3.20 |
| 47.50 | Aid Station 10 | 8:45pm | ● | ● | ● | ? | 2.60 |
| 50.10 | 50 Mile Finish | 10:45pm | ● | ? | ? | ? | ? |
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